PXD064852

PXD064852 is an original dataset announced via ProteomeXchange.

Title	IntS11 is a totipotent regulator that controls zygotic genome activation
Description	As the first transcriptional milestone in life, zygotic genome activation (ZGA) driven by pioneer factors is a hallmark of totipotency, enabling embryos to transition from maternal to zygotic control. Yet, the mechanisms governing the totipotent state of embryonic chromatin remain a central unresolved question in developmental biology. Here we identified IntS11, a Pol II-associated protein, as a critical totipotency factor in Drosophila. We demonstrate that maternal IntS11 controls totipotent state of chromatin, ensuring proper ZGA by coordinating the transcription machinery assembly. Specifically, IntS11 mediates de novo Pol II recruitment and directs pioneer factors Zelda and GAF to zygotic gene regulatory regions. Our findings unveil a fundamental principle linking IntS11, pioneer factor binding, and Pol II engagement, providing novel insights into orchestration of ZGA.
HostingRepository	iProX
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2026-03-30_02:11:59.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoqiang Zhang
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-10 20:12:41	ID requested
⏵ 1	2026-03-30 02:12:00	announced

Dataset with its publication pending

submitter keyword: Drosophila, embryo, ZGA

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ynu.edu.cn
lab head
Guoqiang Zhang
contact affiliation	Institute of biomedical research, yunnan university
contact email	gqzhang@ynu.edu.cn
dataset submitter

iProX dataset URI