PXD064843

PXD064843 is an original dataset announced via ProteomeXchange.

Title	Identification of a regulatory cascade that activates chloramphenicol biosynthesis in Streptomyces venezuelae
Description	Specialised metabolites made by the genus Streptomyces form the basis of 55% of clinically used antibiotics as well as cancer therapeutics, immunosuppressants and other anti-infectives. Streptomyces venezuelae is a model for the genus because it is fast-growing, sporulates in liquid culture within 24 hours and coordinates production of the antibiotic chloramphenicol with sporulation. In this work we demonstrate that chloramphenicol biosynthesis is controlled by a regulatory cascade involving the developmental transcription factors OrrA, WblA and AdpA. OrrA directly activates the production of the WhiB-family protein WblA which binds to 90 target gene promoters, including adpA. AdpA is a pleiotropic regulator that directly activates chloramphenicol biosynthesis, and we propose that deletion of orrA or wblA results in an increase in the levels of AdpA which in turn leads to the increase in chloramphenicol biosynthesis observed in these mutants.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_10:28:43.709.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD064843
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Streptomyces venezuelae; NCBI TaxID: NEWT:54571;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-10 11:14:23	ID requested
⏵ 1	2026-05-21 10:28:44	announced

10.6019/PXD064843;

10.1099/MIC.0.001706;

submitter keyword: Streptomyces, venezuelae, OrrA, WblA

Matt Hutchings
contact affiliation	John Innes Centre Dept. Molecular Microbiology Norwich Research Park Norwich, UK
contact email	matt.hutchings@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064843
     1. Label: PRIDE project
     2. Name: Identification of a regulatory cascade that activates chloramphenicol biosynthesis in Streptomyces venezuelae