PXD064839

PXD064839 is an original dataset announced via ProteomeXchange.

Title	Persistence of Yersinia pestis in tap water microcosm.
Description	This study investigates the long-term persistence of Yersinia pestis in tap water microcosms at 25°C and examines its proteomic landscape and ability to cause lethal infection in a mouse model during persistence, assessing its potential risk for waterborne transmission. Five Y. pestis strains representing different biovars and molecular profiles were studied by seeding tap water microcosms with different bacterial concentrations. All strains remained viable for approximately 120 days in High-Concentration Microcosms (HCM) and 60 days in Low-Concentration Microcosms (LCM). Mass spectrometry analysis revealed that 1,825 proteins (~72%) remained expressed in tap water for over 100 days, with 113 and 43 proteins showing differential expression at 60 and 100 days in HCM, respectively, suggesting adaptive shifts to the aquatic environment. Stress-response proteins (CspA1, CspA2, HspQ) and regulatory proteins (LysR, GntR) appeared to support survival, while virulence-associated proteins (YopE, YopB, ail, adhesins) were also expressed. Notably, mice inoculated with water from day 90 HCM succumbed to infection, underscoring the pathogen’s ability to retain infectivity even in unfavourable conditions. These findings highlight the resilience of Y. pestis in water, its prolonged survival while maintaining virulence, and the potential risks of waterborne transmission, emphasizing the need for effective mitigation strategies against biothreat agents.
HostingRepository	PRIDE
AnnounceDate	2026-01-14
AnnouncementXML	Submission_2026-01-13_23:54:18.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Subodh Kumar
SpeciesList	scientific name: Yersinia pestis; NCBI TaxID: NEWT:632;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-10 09:29:12	ID requested
⏵ 1	2026-01-13 23:54:19	announced

10.1007/s00284-025-04583-6;

Rani P, Kumar S, A Proteomic View on Persistence of Yersinia pestis in Tap Water Microcosm. Curr Microbiol, 82(12):598(2025) [pubmed]

submitter keyword: Culturability

Proteomic adaptations

Tap-water microcosm

Yersinia pestis.

Dr. Subodh Kumar
contact affiliation	Defence Research & Development Establishment, Gwalior.
contact email	subodh.drde@gov.in
lab head
Subodh Kumar
contact affiliation	Defence Research & Development Establishment, Gwalior
contact email	subodh.drde@gov.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD064839

PRIDE project URI

• PRIDE
  1. PXD064839
     1. Label: PRIDE project
     2. Name: Persistence of Yersinia pestis in tap water microcosm.