PXD064837

PXD064837 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics analysis of Complement Factor H and its effect on complement regulatory function during Streptococcus pneumoniae-associated hemolytic uremic syndrome
Description	Hemolytic uremic syndrome caused by an invasive Streptococcus pneumoniae infection (SP-HUS) is a rare and severe disease that primarily affects children under two years of age. The pathophysiology of SP-HUS remains poorly understood, and treatment is largely supportive. Complement factor H (FH) is a key regulator of the alternative pathway of the complement system. It has been hypothesized that loss of sialic acids from FH’s N-glycans may impair its regulatory functions, thereby potentially leading to complement-mediated endothelial cell damage in SP-HUS. In this study, we investigated the N-glycosylation patterns of FH across three N-glycosylation sites for four SP-HUS patients and compared it to healthy controls using LC-MS/MS-based glycopeptide profiling.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_05:52:20.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hans Wessels
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	complex glycosylation; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-10 08:54:00	ID requested
⏵ 1	2025-08-21 05:52:20	announced

10.3389/FIMMU.2025.1645196;

submitter keyword: hemolytic uremic syndrome, glycosylation, Streptococcus pneumoniae, glycoproteomics,Complement Factor H, functional assays

Hans JCT Wessels
contact affiliation	Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands
contact email	hans.wessels@radboudumc.nl
lab head
Hans Wessels
contact affiliation	Radboudumc
contact email	hans.wessels@radboudumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064837
     1. Label: PRIDE project
     2. Name: Glycoproteomics analysis of Complement Factor H and its effect on complement regulatory function during Streptococcus pneumoniae-associated hemolytic uremic syndrome