PXD064815

PXD064815 is an original dataset announced via ProteomeXchange.

Title	Proteomic signatures of the chemotherapy-induced damage in the human ovary
Description	Although the mechanisms underlying chemotherapy-induced damage to germ cells are being increasingly deciphered, its impact on the ovarian microenvironment remains largely unexplored. The ovarian stroma is equally exposed to chemotherapy, and since its cells and components are in active communication with follicles, any microenvironmental changes induced by chemotherapy might affect follicles as well. The present project aimed to characterise the alterations of the human ovarian microenvironment and to comprehensively elucidate the mechanisms of ovarian injury in response to chemotherapy. Human ovarian fragments from women previously exposed or not to first-line chemotherapy were analysed using mass spectrometry and bioinformatics. A total of 5,209 proteins were detected in both chemotherapy-exposed and control ovaries. Comparative proteomic analysis revealed 237 differentially expressed proteins (4.5%) post-treatment, among which 162 were upregulated and 75 were downregulated in ovarian cells from chemotherapy-treated patients compared to controls. Biological pathways related to immune response, hypoxia and apoptosis were upregulated following chemotherapy exposure, while those involved in cell cycle and DNA repair were downregulated. Markers of the ECM network were also dysregulated. These findings provide evidence that chemotherapy significantly alters the ovarian stroma, emphasizing the need to further investigate the long-term consequences for fertility and reproductive lifespan after transplantation. They also suggest target proteins which may drive these chemotherapy-associated ovarian damage for future pharmacoprotection investigations.
HostingRepository	PRIDE
AnnounceDate	2025-11-07
AnnouncementXML	Submission_2025-11-07_07:55:24.935.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xavier Bisteau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-10 01:57:06	ID requested
⏵ 1	2025-11-07 07:55:25	announced

10.1093/HUMREP/DEAF203;

submitter keyword: Ovarian tissue, gonadotoxicity, ovarian microenvironment, chemotherapy, stromal cells, fertility preservation

Isabelle Demeestere
contact affiliation	Research Laboratory in Human Reproduction, Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium
contact email	isabelle.demeestere@ulb.be
lab head
Xavier Bisteau
contact affiliation	IRIBHM J.E Dumont, Faculty of Medicine, Université Libre de Bruxelles
contact email	xavier.bisteau@ulb.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD064815

PRIDE project URI

• PRIDE
  1. PXD064815
     1. Label: PRIDE project
     2. Name: Proteomic signatures of the chemotherapy-induced damage in the human ovary