PXD064796

PXD064796 is an original dataset announced via ProteomeXchange.

Title	Mouse T-cell LC-MSMS from WT and MTDH KO
Description	This study uncovers host Metadherin (MTDH) as a pivotal regulator of systemic tumor progression, orchestrating liver metabolism and CD8⁺ T cell immunity. Tumor-derived extracellular vesicles prompt Kupffer cells to release TNFα and TGF-β, which, through MTDH-driven NF-κB signaling, suppress hepatic PPARα activity and lipid oxidation. The resulting systemic lipid accumulation hampers CD8⁺ T cell functionality. Eliminating MTDH in both hepatocytes and CD8⁺ T cells restores lipid metabolism, enhances mitochondrial health, and strengthens antitumor immunity. Proteomic profiling of tumor-infiltrating CD8⁺ T cells highlights increased OXPHOS activity, mitochondrial biogenesis, and diminished ferroptosis markers in MTDH-deficient cells. Combining MTDH inhibition with anti-PD-1 therapy amplifies immunotherapeutic effectiveness, offering a promising metabolic reprogramming strategy.
HostingRepository	PRIDE
AnnounceDate	2026-08-29
AnnouncementXML	Submission_2026-08-29_07:41:48.301.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ji Woo Park
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-09 18:20:23	ID requested
⏵ 1	2026-08-29 07:41:49	announced

Tang Y, Chen Z, Sarkar H, Wei Y, Lopez-Darwin SL, Roichman A, Park JW, Li Q, Fang C, Chen Z, Gow J, Shen M, Shi J, Jiang L, Klar R, Hang X, Lugo JR, DeCoste CJ, Miller JM, Wang W, Romanienko P, Welm AL, Cristea IM, Wan L, Rabinowitz JD, Kang Y, T cell immunity to promote tumor progression. Cell Metab, ():(2026) [pubmed]

10.1016/j.cmet.2026.08.003;

submitter keyword: Mouse, timsTOF Ultra, tumor, T-cell

Ileana M. Cristea
contact affiliation	Department of Molecular Biology, Cristea Lab, Princeton University, Princeton, NJ, US (lab head)
contact email	icristea@princeton.edu
lab head
Ji Woo Park
contact affiliation	Princeton University
contact email	jp1381@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD064796

PRIDE project URI

• PRIDE
  1. PXD064796
     1. Label: PRIDE project
     2. Name: Mouse T-cell LC-MSMS from WT and MTDH KO