PXD064793

PXD064793 is an original dataset announced via ProteomeXchange.

Title	Hybrid CCM in Thalassiosira pseudonana
Description	Manipulated carbonate chemistry to disentangle proteins involved in the carbon concentrating mechanism (CCM) of Thalassiosira pseudonana from other cellular mechanisms that employ the same proteins. The malate/oxaloacetate shuttle shares overlap with biochemical CCMs while also supporting pH regulation, maintenance of redox poise, cyclic electron transport, and the dissipation of excess light energy. Three treatments were used to separate out the effects of pH and pCO2: low pCO2/high pH (LCHpH), high pCO2/low pH (HCLpH), and low pCO2/low pH (LCLpH). Results from this analysis were consistent with a hybrid CCM within T. pseudonana that consists of both biophysical and biochemical steps. No evidence for alternative cellular mechanisms that could explain protein abundance profiles observed under low pCO2 conditions was found.
HostingRepository	PRIDE
AnnounceDate	2025-10-13
AnnouncementXML	Submission_2025-10-13_06:41:12.501.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anthony Himes
SpeciesList	scientific name: Thalassiosira pseudonana CCMP1335; NCBI TaxID: NEWT:296543;
ModificationList	monohydroxylated residue; N6-lipoyl-L-lysine; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-09 14:44:50	ID requested
⏵ 1	2025-10-13 06:41:13	announced

10.1093/JXB/ERAF450;

submitter keyword: Thalassiosira pseudonana, carbon concentrating mechanism, hybrid CCM

Adam Kustka
contact affiliation	Earth and Enviornmental Sciences; Rutgers University-Newark; Newark, NJ, USA
contact email	adam.kustka@rutgers.edu
lab head
Anthony Himes
contact affiliation	Rutgers University-Newark
contact email	anthony.himes@rutgers.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064793
     1. Label: PRIDE project
     2. Name: Hybrid CCM in Thalassiosira pseudonana