PXD064787

PXD064787 is an original dataset announced via ProteomeXchange.

Title	Development of Cellular Energy Metabolism During Differentiation of Human iPSCs Into Cortical Neurons
Description	We performed label-free quantitative proteomic profiling to characterize metabolic remodeling during early differentiation of human iPSCs into excitatory cortical neurons induced by NGN2 overexpression. Proteomic analysis was conducted at three time-points (iPSCs, day 7, and day 14 post-induction), focusing on neuronal, mitochondrial, and biosynthetic pathways. Our findings identify the first week of neuronal differentiation as a critical period characterized by coordinated proteomic and metabolic reprogramming, including the suppression of pluripotency markers, acquisition of neuronal identity, and reduced proliferation. Mitochondrial biogenesis is initiated during this phase, accompanied by the upregulation of oxidative phosphorylation and fatty acid oxidation. These data establish a proteomic baseline for cortical neuron differentiation and provide a reference platform for the interpretation of metabolic alterations in patient-derived models.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-23_16:35:18.575.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Vrbacky
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-09 12:15:40	ID requested
⏵ 1	2025-11-23 16:35:19	announced

10.1007/s12035-025-05284-8;

Dana, č, í, kov, á Š, Pecina P, Pecinov, á A, Svoboda J, Vondr, á, š, ek D, Basello DA, Č, ajka T, Hadraba D, Mr, á, č, ek T, Ko, ř, í, nek V, Ot, á, hal J, Development of Cellular Energy Metabolism During Differentiation of Human iPSCs into Cortical Neurons. Mol Neurobiol, 63(1):37(2025) [pubmed]

submitter keyword: Human, LC-MSMS, iPSCs, cell culture, Neurons

Jakub Otahal
contact affiliation	Department of Pathophysiology, Second Faculty of Medicine, Charles University in Prague
contact email	jakub.otahal@lfmotol.cuni.cz
lab head
Marek Vrbacky
contact affiliation	Czech Academy of Sciences
contact email	proteom.krc@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD064787

PRIDE project URI

• PRIDE
  1. PXD064787
     1. Label: PRIDE project
     2. Name: Development of Cellular Energy Metabolism During Differentiation of Human iPSCs Into Cortical Neurons