PXD064756

PXD064756 is an original dataset announced via ProteomeXchange.

Title	The melanoma cell line RPMI-7951 adhesome in long-term culture and its comparison to the adhesome upon integrin αV knockdown
Description	Integrins are involved in the metastatic cascade and have been found to be responsible for intrinsic and acquired therapy resistance. Therefore, targeting integrins, or more specifically the signal transduction via integrin adhesion complexes (IACs), is a promising therapeutic opportunity to reduce tumor metastasis and overcome therapeutic resistance. Using mass spectrometry–based proteomics, we analysed the components of integrin adhesion complexes of RPMI-7951 cells in long term culture. The adhesome of RPMI-7951 cells contained three integrin receptor subunits: αV, β5 and β1. The most abundant integrin subunits were αV and β5, suggesting that these cells primarily use αVβ5 for adhesion in long term 2D culture. Integrin subunit α5 was detected, but at very low levels, suggesting that these cells may also express α5β1. To determine which proteins were found in αVβ5-containing adhesions, transient knockdown of integrin subunit αV was performed and IAC composition was compared to cells transfected with control siRNA.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:18:39.710.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikolina Stojanovic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-08 22:25:24	ID requested
⏵ 1	2026-04-26 16:18:40	announced

10.1186/s12964-026-02771-w;

Stojanovi, ć N, Rac A, Lon, č, ari, ć M, Tadijan A, Parad, ž, ik M, Acman M, Humphries JD, Humphries MJ, Ambriovi, ć, -Ristov A, KANK2 at focal adhesions regulates their maintenance and dynamics, while at fibrillar adhesions it influences cell migration via microtubule-dependent mechanism. Cell Commun Signal, 24(1):(2026) [pubmed]

submitter keyword: None

Andreja Ambriović-Ristov
contact affiliation	Laboratory for Cell Biology and Signalling, Division of Molecular Biology, Ruder Boskovic Institute, Zagreb, Croatia
contact email	Andreja.Ambriovic.Ristov@irb.hr
lab head
Nikolina Stojanovic
contact affiliation	Ruđer Bošković Institute
contact email	nikolina.stojanovic@irb.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD064756

PRIDE project URI

• PRIDE
  1. PXD064756
     1. Label: PRIDE project
     2. Name: The melanoma cell line RPMI-7951 adhesome in long-term culture and its comparison to the adhesome upon integrin αV knockdown