PXD064752

PXD064752 is an original dataset announced via ProteomeXchange.

Title	Quantitative interactome profiling of rabies virus P-protein isoforms P1 and P3
Description	RNA viruses must achieve complex replication and immune evasion functions using limited coding capacity. The rabies virus P protein is a multifunctional viral protein expressed in multiple isoforms via alternative translation. In this study, we investigate how structural and functional diversity arises between two isoforms—P1 (full-length) and P3 (a naturally truncated form)—and how these differences influence host protein interactions. We used GFP-tagged P1 and P3 proteins expressed in HEK293T cells to perform quantitative immunoprecipitation-mass spectrometry (IP-MS) and define isoform-specific interactomes. This dataset includes raw and processed IP-MS data for GFP-P1, GFP-P3, and GFP control (each in quadruplicate), and supports the conclusion that conformational and functional diversification of viral proteins enables expanded host interface capacity. This study provides new mechanistic insights into how viruses exploit isoform diversity and RNA-driven interactions to overcome genome limitations.
HostingRepository	PRIDE
AnnounceDate	2025-10-10
AnnouncementXML	Submission_2025-10-09_16:58:49.906.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephen Rawlinson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Lyssavirus rabies; NCBI TaxID: NCBITaxon:11292;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-08 18:21:13	ID requested
⏵ 1	2025-10-09 16:58:50	announced

Dataset with its publication pending

submitter keyword: isoform specificity, membraneless organelles, proteomics, LLPS, mass spectrometry, P protein, IP-MS, viral-host interactions, immunoprecipitation,Rabies virus, interactome

Gregory W. Moseley
contact affiliation	Monash University, Clayton, Australia
contact email	greg.moseley@monash.edu
lab head
Stephen Rawlinson
contact affiliation	Monash University
contact email	stephen.rawlinson@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD064752

PRIDE project URI

• PRIDE
  1. PXD064752
     1. Label: PRIDE project
     2. Name: Quantitative interactome profiling of rabies virus P-protein isoforms P1 and P3