PXD064751

PXD064751 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of MYDGF knockdown in lung adenocarcinoma cells
Description	Lung adenocarcinoma (LUAD) remains the leading cause of cancer-related mortality worldwide. Unlike patients harboring epidermal growth factor receptor (EGFR) mutations, those with wild-type EGFR lack effective targeted therapeutic options. We established a super-SILAC-based proteomic dataset (PXD046807) comprising LUAD tissues stratified by EGFR mutation status and clinical stages. Notably, myeloid-derived growth factor (MYDGF) was identified as a potential key regulator in patients with wild-type EGFR LUAD. To further investigate the role of MYDGF in LUAD, we applied gene knockdown combined with tandem mass tag (TMT)-based quantitative proteomic analysis to search for the specific biological processes regulated by MYDGF in normal lung fibroblasts and LUAD cells with different EGFR status.
HostingRepository	PRIDE
AnnounceDate	2025-06-22
AnnouncementXML	Submission_2025-06-21_22:43:26.447.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chia-Jung Yu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-08 18:20:52	ID requested
⏵ 1	2025-06-21 22:43:26	announced

Dataset with its publication pending

submitter keyword: MYDGF, lung adenocarcinoma, knockdown, tandem mass tags (TMT)

Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@gmail.com
lab head
Chia-Jung Yu
contact affiliation	Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan
contact email	yucj1124@mail.cgu.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD064751

PRIDE project URI

• PRIDE
  1. PXD064751
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of MYDGF knockdown in lung adenocarcinoma cells