PXD064749 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype |
| Description | The X chromosome inactivation (XCI) is induced by Xist long non-coding RNA and protein-coding genes. However, the small non-coding RNA function in XCI remains unidentified. Our genome-wide, loss-of-function CRISPR/Cas9 screen in female fibroblasts examines the role of microRNA (miRNAs) in XCI. A striking finding is the identification of miR106a among the top candidates from 46 the screen. Loss of miR106a is accompanied by altered Xist interactome, leading to dissociation and destabilization of Xist. The XCI interference via miR106a inhibition has therapeutic implications for Rett syndrome (RTT) girls with a defective X-linked MECP2 gene. Here, we discovered that the genetic inhibition of miR106a significantly improves several facets of RTT pathology: it increases the life span, enhances locomotor activity and exploratory behavior, and diminishes breathing variabilities. Our results suggest that miR106a targeting offers a feasible therapeutic strategy for RTT and other monogenic X-linked neurodevelopmental disorders. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-09 |
| AnnouncementXML | Submission_2026-02-08_18:21:14.221.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD064749 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Robert Maxwell |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-08 12:21:34 | ID requested | |
| ⏵ 1 | 2026-02-08 18:21:15 | announced | |
Publication List
| Lou S, DJiake Tihagam R, Wasko UN, Equbal Z, Venkatesan S, Braczyk K, Przanowski P, Il Koo B, Saltani I, Singh AT, Likhite S, Powers S, Souza GMPR, Maxwell RA, Yu J, Zhu LJ, Beenhakker M, Abbott SBG, Lu Z, Green MR, Meyer KC, Tushir-Singh J, Bhatnagar S, Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype. Nat Commun, 16(1):6169(2025) [pubmed] |
| 10.1038/s41467-025-61092-7; |
| 10.6019/PXD064749; |
Keyword List
| submitter keyword: LCMS,Mus Mus, ChIRP-MS |
Contact List
| Sanchita Bhatnagar |
|---|
| contact affiliation | Department of Medical Microbiology and Immunology, the University of California Davis Schoolof Medicine, 1275 Health Sciences Drive, Tupper Hall 3134, Davis, CA 95616, USA. Tel: 530-754-3074 |
| contact email | sbbhatnagar@ucdavis.edu |
| lab head | |
| Robert Maxwell |
|---|
| contact affiliation | UCB |
| contact email | robmaxwell@berkeley.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064749 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064749
- Label: PRIDE project
- Name: Targeting microRNA-dependent control of X chromosome inactivation improves the Rett Syndrome phenotype
