PXD064745

PXD064745 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation site identifcaiton of OsHSFA4d
Description	To identify the residues of OsHSFA4d phosphorylated by OsCDPK24, the in vitro kinase assays were conducted by incubating recombinant MBP-HA-OsCDPK24 + GST-OsHSFA4d and MBP-HA + GST-OsHSFA4d. The reaction was terminated by adding SDS loading buffer to a 1×concentration, and the reaction products were subjected to CBB staining and immunoblot analysis as described above. The CBB-stained protein bands corresponding to phosphorylated OsHSFA4d were excised and subjected to LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_19:43:13.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiang Lu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-07 22:59:48	ID requested
⏵ 1	2025-06-08 19:43:13	announced

Dataset with its publication pending

submitter keyword: Phosphorylation site

OsHSFA4d

Xuewei Chen
contact affiliation	New Cornerstone Science Laboratory, State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
contact email	xwchen88@163.com
lab head
Xiang Lu
contact affiliation	State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China
contact email	luxiang@sicau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD064745

PRIDE project URI

• PRIDE
  1. PXD064745
     1. Label: PRIDE project
     2. Name: Phosphorylation site identifcaiton of OsHSFA4d