PXD064721 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | pulsed-SILAC after 55LCC depletion |
| Description | To study the effect of individual protein depletion in 55LCC, we generated CRISPR-based FKBP12F36V-tagged HCT116 cell lines for the following 55LCC members: SPATA5 (Q8NB90) and SPATA5L1 (Q9BVQ7). CINP (Q9BW66), and C1orf109 (Q9NX04). We then performed several characterization experiments during depletion (50nM of the dTAGV-1 PROTAC molecule or an equimolar DMSO solvent control) for the indicated times. Here, we focus on the abundance of newly synthesized proteins. We identified these proteins via the incorporation of heavy-labeled amino acids (‘Heavy’ L-Arginine-13C61H1415N416O2, ‘Heavy’ L-Lysine-13C61H1415N216O2) within a fixed pulse for 24 hours after diverse pretreatments, as indicated in the manuscript and specified in the uploaded samples overview sheet (Experimental_Design). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-29 |
| AnnouncementXML | Submission_2026-07-29_11:38:26.555.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jonas Walter |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-06-06 04:24:02 | ID requested | |
| ⏵ 1 | 2026-07-29 11:38:27 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SPATA5L1, C1ORF109,55LCC, CINP, SPATA5 |
Contact List
| Chunaram Choudhary |
|---|
| contact affiliation | university of copenhagen, medical faculty, novo nordisk center for protein research, proteomics program, cell signaling lab |
| contact email | chuna.choudhary@cpr.ku.dk |
| lab head | |
| Jonas Walter |
|---|
| contact affiliation | University of Bonn |
| contact email | jonaswalter@posteo.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD064721 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064721
- Label: PRIDE project
- Name: pulsed-SILAC after 55LCC depletion
