PXD064705

PXD064705 is an original dataset announced via ProteomeXchange.

Title	Multi-Omics Analysis Reveals RNA Polymerase II Degradation as a Novel Mechanism of PF-3758309's Anti-Tumor Activity
Description	In this study, we conducted a comprehensive multi-omics analysis, including proteomics, transcriptomics, and ubiquitinomics, to investigate the mechanism of PF-3758309 in HCT116 cells. Our results revealed that PF-3758309 promotes the degradation of RNA polymerase II subunit proteins (POLR2A/B/E) via the cullin-RING ligase pathway.
HostingRepository	iProX
AnnounceDate	2025-06-05
AnnouncementXML	Submission_2025-08-26_20:20:56.513.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinglong Jia
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	6x(13)C labeled L-lysine; TMT6plex reporter fragment; ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-05 23:45:48	ID requested
1	2025-06-05 23:46:08	announced
⏵ 2	2025-08-26 20:20:57	announced	2025-08-27: Add info.

Jia X, Zhang J, Pan L, He J, Zhu M, Zhao L, Zhang X, Zhao W, Xie D, Shen X, Liu B, Tan M, Multi-omics analysis reveals RNA polymerase II degradation as a novel mechanism of PF-3758309's anti-tumor activity. Cell Death Discov, 11(1):404(2025) [pubmed]

submitter keyword: PF-3758309, POLR2A/B/E, DDB2, MLN4924, Ubiquitin-proteasome system, Anti-tumor activity

Minjia Tan
contact affiliation	Shanghai institute of Materia Medica Chinese Academy of Sciences
contact email	Tanminjia@outlook.com
lab head
Xinglong Jia
contact affiliation	Shanghai institute of Materia Medica Chinese Academy of Sciences
contact email	jiaxlzzu@163.com
dataset submitter

iProX dataset URI