PXD064674

PXD064674 is an original dataset announced via ProteomeXchange.

Title	Steric Repulsion Counteracts ER-to-Lipid Droplet Protein Movement
Description	Lipid droplets (LDs) are organelles with a neutral lipid core surrounded by a phospholipid monolayer continuous with the endoplasmic reticulum’s (ER) cytosolic leaflet. LDs' dynamics and function relate closely to their constantly remodeling proteome composition. Key proteins relocate from the ER to LDs, yet the mechanisms governing their movement and accumulation in LDs remain poorly understood. Here, we developed an innovative ex cellulo tool to classify ER proteins based on their affinity for LDs. We identified steric hindrance as a prime factor regulating ER-to-LD protein transfer, where proteins with higher LD affinity can effectively displace those with lower affinity from the LD surface. Consistent with this model, we observed that the differentiation of 3T3 pre-adipoc tes into adipocytes involves extensive remodeling of ER proteins targeting LDs, with Plin1—a high-affinity LD protein—becoming predominantly recruited, correlating with a reduction in the recruitment of other ER proteins. These findings highlight lateral protein-protein exclusion as a fundamental mechanism in shaping the LD proteome.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_16:19:46.363.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064674
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bianca Esch
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-05 04:01:19	ID requested
⏵ 1	2025-09-28 16:19:47	announced

10.1126/sciadv.adu6998;

Damm A, Omrane M, Stojanovi, ć O, Esch BM, Zouiouich M, Carpentier M, Klemm R, Fr, ö, hlich F, For, ê, t L, Thiam AR, Steric repulsion counteracts ER-to-lipid droplet protein movement. Sci Adv, 11(39):eadu6998(2025) [pubmed]

10.6019/PXD064674;

submitter keyword: LC-MS/MS

Florian Fröhlich
contact affiliation	Osnabrück University Department of Biology/Chemistry, Bioanalytical Chemistry section, 49076 Osnabrück, Germany
contact email	florian.froehlich@uni-osnabrueck.de
lab head
Bianca Esch
contact affiliation	Osnabrück University
contact email	bianca.esch@uos.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064674

PRIDE project URI

• PRIDE
  1. PXD064674
     1. Label: PRIDE project
     2. Name: Steric Repulsion Counteracts ER-to-Lipid Droplet Protein Movement