PXD064673

PXD064673 is an original dataset announced via ProteomeXchange.

Title	Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication
Description	The influenza A virus polymerase, consisting of a heterotrimer of three viral proteins, carries out both transcription and replication of the viral RNA genome. These distinct activities are regulated by viral proteins that vary in abundance during infection, and by various co-opted host cell proteins, which serve as targets for the development of novel antiviral interventions. However, little is known about which host proteins direct transcription and which replication. In this report, we performed a differential interactome screen to identify host proteins co-opted as either transcription- or replication-specific factors. We found that distinct sets of host proteins interact with the influenza polymerase as it carries out the different activities. We functionally characterised HMGB2 and RUVBL2 as replication-specific cofactors and RPAP2 as a transcription-specific cofactor. Our data demonstrate that comparative proteomics can be used as a targeted approach to uncover virus-host interactions that regulate specific stages of the viral lifecycle.
HostingRepository	PRIDE
AnnounceDate	2026-02-04
AnnouncementXML	Submission_2026-02-04_04:17:11.585.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Skehel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-05 03:55:24	ID requested
⏵ 1	2026-02-04 04:17:12	announced

Dataset with its publication pending

submitter keyword: influenza virus polymerase, transcription, comparative proteomics, antiviral targets, replication,Host-pathogen interactions

Wendy S. Barclay
contact affiliation	Department of Infectious Disease, Faculty of Medicine, Imperial College London, London, United Kingdom.
contact email	w.barclay@imperial.ac.uk
lab head
Mark Skehel
contact affiliation	Francis Crick Institute
contact email	skehelm@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064673

PRIDE project URI

• PRIDE
  1. PXD064673
     1. Label: PRIDE project
     2. Name: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication