PXD064612

PXD064612 is an original dataset announced via ProteomeXchange.

Title	Limited proteolysis of SARS-CoV-2 Spike treated at pH 2 or pH 7
Description	SARS-CoV-2 is a respiratory virus spreading via aerosol particles. Aerosol particles can exhibit extreme environmental conditions affecting contained viruses, including acidic pH. Here, we study how harsh pH conditions, such as pH 2, can affect the structure of Spike, which is the main protein mediating viral entry into host cells. By performing limited proteolysis with the unspecific protease K coupled to mass spectrometry, we identified 4 semi-tryptic peptides in the S2 subdomain of spike. These results indicate structural changes in the S2 subdomain induced by treatment at pH 2.
HostingRepository	PRIDE
AnnounceDate	2025-07-17
AnnouncementXML	Submission_2025-07-17_05:50:39.707.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Irina Glas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; ACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-04 02:00:40	ID requested
⏵ 1	2025-07-17 05:50:40	announced

10.1038/S42003-025-08514-W;

submitter keyword: SARS-CoV-2, LC-MS, Spike, acidic pH, LiP

Silke Stertz
contact affiliation	Institute of Medical Virology, University of Zurich, Switzerland
contact email	stertz.silke@virology.uzh.ch
lab head
Irina Glas
contact affiliation	Institute of Medical Virology, University of Zurich
contact email	irkaglas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD064612

PRIDE project URI

• PRIDE
  1. PXD064612
     1. Label: PRIDE project
     2. Name: Limited proteolysis of SARS-CoV-2 Spike treated at pH 2 or pH 7