PXD064597

PXD064597 is an original dataset announced via ProteomeXchange.

Title	Systematic Analysis of C-termini of Small Open Reading Frames Encoded Peptides in Human Cancer Cells
Description	The C-terminal of proteins regulates their biological functions. Small open reading frame-encoded peptides (SEPs) are a novel class of gene expression products that participate in various biological activities. The C-termini of SEPs have also been found to affect their function, but the polymorphism of SEPs' C-terminal has not yet been systematically elucidated. Here, we utilized C-terminal proteomics technology to systematically analyze the C-termini of SEPs in human tumor cell lines. We combined two SEPs enrichment methods (ACN and heat precipitation) and two C-terminal peptides enrichment methods (MOLEX and CTAILS) to identified the C-terminal peptides of SEPs. Each experiment was performed with triplicate.
HostingRepository	iProX
AnnounceDate	2025-06-03
AnnouncementXML	Submission_2025-06-03_23:44:26.744.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingbo Peng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 23:44:09	ID requested
⏵ 1	2025-06-03 23:44:27	announced

Dataset with its publication pending

submitter keyword: C-terminomics, sORF-encoded peptides, truncate, proteolytic

alternative splicing

Cuihong Wan
contact affiliation	Central China Normal University
contact email	ch_wan@ccnu.edu.cn
lab head
Mingbo Peng
contact affiliation	Central China Normal University
contact email	1241677348@qq.com
dataset submitter

iProX dataset URI