PXD064586

PXD064586 is an original dataset announced via ProteomeXchange.

Title	Multi-omics analysis of arboviral capsid targets in mosquitoes reveals a pro-viral function of the chromatin-remodeling Brahma complex
Description	In recent years, arbovirus cases have surged dramatically due to the expanding distribution of the main arbovirus-vectors Aedes and Culex mosquitoes. Despite significant efforts to uncover Orthoflavivirus-specific host adaptation mechanisms of humans, systematic studies aiming to characterize virus–host interactions in arthropods are largely missing. The capsid protein is highly versatile, with multiple functions and subcellular localizations, making it an attractive target for studying host susceptibility, vector competence, and virus–vector adaptation. Here, we employed affinity purification coupled to mass spectrometry to systematically evaluate the ability of capsid proteins of 12 different pathogenic arboviruses spanning three genera to interact with the Ae. aegypti proteome. This extensive protein-protein interaction atlas uncovered novel host targets, implicated in diverse cellular pathways i.e. translation, apoptosis and RNA processing. To functionally characterize prioritized interacting host proteins, we systematically silenced 110 host genes in Ae. aegypti-derived cells and characterized their impact on the replication of three representative arboviruses: La Crosse virus, dengue virus and West Nile virus. This approach identified several novel host-dependency factors, including a new role for two components of the chromatin-remodeling Brahma complex. Interestingly, systematic silencing of individual Brahma BAP subunits revealed a conserved pan-orthoflavivirus role for both BAP and PBAP subcomplexes, when compared to prototypic alphaviruses. Furthermore, using a combination of biochemical and sequencing approaches, we characterized the cellular determinants of these interactions and profiled their functional consequences on the chromatin landscape. Altogether, this study provides an evidence-based repository to categorize and characterize novel arboviral capsid targets and explore arbovirus-vector interactions.
HostingRepository	PRIDE
AnnounceDate	2026-02-07
AnnouncementXML	Submission_2026-02-06_21:51:55.265.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Flaviviridae; NCBI TaxID: NEWT:11050; scientific name: Orthoflavivirus; NCBI TaxID: NEWT:3044782; scientific name: Aedes aegypti; NCBI TaxID: NEWT:7159; scientific name: Alphavirus; NCBI TaxID: NEWT:11019; scientific name: Orthobunyavirus; NCBI TaxID: NEWT:11572;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 19:44:33	ID requested
⏵ 1	2026-02-06 21:51:56	announced

10.1016/J.MCPRO.2026.101512;

submitter keyword: None

Pietro Scaturro
contact affiliation	Leibniz Institute of Virology (LIV), Hamburg, Germany
contact email	pietro.scaturro@leibniz-liv.de
lab head
Pietro Scaturro
contact affiliation	Systems Arbovirology, Leibniz Institute of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064586

PRIDE project URI

• PRIDE
  1. PXD064586
     1. Label: PRIDE project
     2. Name: Multi-omics analysis of arboviral capsid targets in mosquitoes reveals a pro-viral function of the chromatin-remodeling Brahma complex