PXD064569

PXD064569 is an original dataset announced via ProteomeXchange.

Title	Reduced collagen degradation in pelvic urine precedes kidney fibrosis induced by unilateral ureteral obstruction
Description	Kidney fibrosis is a major hallmark of chronic kidney disease and is defined by an excessive accumulation of extracellular matrix, primarily collagens. While collagen synthesis has been extensively investigated, the contribution of impaired collagen degradation to the development of fibrosis remains poorly understood. Collagen degradation involves the activity of proteases producing collagen-derived peptides (CDPs). Here we tested the hypothesis whether reduced collagen degradation participates to the development of kidney fibrosis. For that, CDPs were quantified in pelvic urine of kidney undergoing fibrosis following unilateral ureteral obstruction (UUO). C57Bl6 male mice underwent UUO- or sham-surgery and were sacrificed after 3- or 7-days (3D or 7D). Fibrosis was evaluated by histology. Urinary CDP composition (qualitative-sequences and quantitative-abundances) was analysed by mass spectrometry (MS). Based on collagen protein deposition, UUO-induced fibrosis was detected at 7D and not at 3D. At both 3D and 7D, of the more than 300 significantly different CDPs were found in UUO-pelvic urine, 70% of them displaying a reduced abundance in UUO compared to sham-bladder. In UUO-bladder urine, significant CDPs were found only at 7D with 79% of them displaying increased abundance. CDPs derived from Col1a1, Col1a2 and Col3a1 were the most represented and distributed in clusters along their parental protein sequence. Most clusters were composed of seemingly decreasing length CDPs containing the entire sequence of the shortest CDP. Within the clusters, inter-CDP correlations of abundance were very different between UUO-pelvis and UUO-bladder at 3D and 7D post-UUO. In conclusion, using a mouse model of fibrosis that enables the specific study of CDPs from the fibrotic kidney, we observe that reduced collagen degradation could play a key role in the early stages of fibrosis development. Our research suggests a potential need for a shift in the development of anti-fibrotic strategies, emphasizing not only the inhibition of collagen synthesis but also the enhancement of its degradation.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_16:23:55.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carine Froment
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 09:06:52	ID requested
⏵ 1	2026-01-11 16:23:56	announced

10.1038/s41598-025-29217-6;

Frattini T, Breuil B, Bul, é, on M, Feuillet G, Chabbert M, Delecroix E, Froment C, Burlet-Schiltz O, Sylla B, Mischak H, Mina IK, Siwy J, Latosinska A, Saulnier-Blache JS, Schanstra J, Reduced collagen degradation in pelvic urine precedes kidney fibrosis induced by unilateral ureteral obstruction. Sci Rep, 15(1):44975(2025) [pubmed]

submitter keyword: chronic kidney disease, kidney fibrosis, urine, Unilateral ureteral obstruction, CE-MS/MS

Joost Schanstra
contact affiliation	Institut National de la Santé et de la Recherche Médicale (INSERM), UMR 1297, Institut des Maladies Métaboliques et Cardiovasculaires, Université de Toulouse, Toulouse, France.
contact email	joost-peter.schanstra@inserm.fr
lab head
Carine Froment
contact affiliation	IPBS-CNRS
contact email	carine.froment@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD064569

PRIDE project URI

• PRIDE
  1. PXD064569
     1. Label: PRIDE project
     2. Name: Reduced collagen degradation in pelvic urine precedes kidney fibrosis induced by unilateral ureteral obstruction