PXD064567

PXD064567 is an original dataset announced via ProteomeXchange.

Title	GMP-compatible extracellular vesicles prevent heart failure after a myocardial infarction through reduction of myofibroblast activation
Description	Ischemic heart disease is the leading cause of heart failure and mortality in the Western world. Survival after a ST-Elevation Myocardial Infarction (STEMI) has not improved over the last decade, mostly because revascularization with percutaneous coronary intervention (PCI) may, paradoxically, induce myocardial ischemia-reperfusion (MIR) injury. The injury is orchestrated by macrophages and leads to myofibroblast activation and additional scar formation. Here, we have developed a good manufacturing practice compatible protocol for the generation of an immune modulatory extracellular vesicle (EV)-rich product from bone marrow mesenchymal stromal. In blinded, placebo-controlled MIR models in mice and pigs, the EV product preserved the myocardial function in the mice hearts and was cardioprotective in the pig models of MIR. To facilitate translation to the clinic, we have also developed a positron emission tomography (PET)-based system to longitudinally detect in vivo heart myofibroblast activation and, thereby, the scar formation in mice and humans. The PET system showed that the EV-product significantly reduced myofibroblast activation and the scar size in placebo-controlled MIR-injury models in mice. In conclusion, PET-directed EV-treatment might offer an individualized additive treatment to PCI in order to prevent heart failure after STEMI.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_04:25:31.794.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pierre Sabatier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 08:48:02	ID requested
⏵ 1	2026-06-12 04:25:32	announced

Dataset with its publication pending

submitter keyword: extracellular vesicles, myocardial infarction

Jesper Olsen
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Pierre Sabatier
contact affiliation	Department of Surgical Sciences, Uppsala University. Novo Nordisk Foundation Center for Protein Research, University of Copenhagen.
contact email	pierre63.sabatier@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD064567

PRIDE project URI

• PRIDE
  1. PXD064567
     1. Label: PRIDE project
     2. Name: GMP-compatible extracellular vesicles prevent heart failure after a myocardial infarction through reduction of myofibroblast activation