PXD064562

PXD064562 is an original dataset announced via ProteomeXchange.

Title	The adaptive molecular landscape to reprogrammed telomeric sequences
Description	Telomeric sequences vary across the tree of life and intimately co-evolve with telomere-binding protein complexes. However, the molecular mechanisms allowing organisms to adapt to new telomeric sequences are difficult to gauge from extant species. Here, we reprogrammed multiple yeast lines to human-like telomeric repeats to unveil their molecular and fitness response to novel telomeres. Using experimental evolution, we observed increased genome instability, proteome remodelling and severe fitness decline, but repeatable mutational events mediate adaptation. These consist in the recurrent amplification of the telomere-binding protein TBF1 by complex aneuploidy or in repeated mutations that attenuate the DNA damage response. Overall, our results outline a response that defines the adaptive molecular landscape to novel telomeric sequences.
HostingRepository	PRIDE
AnnounceDate	2026-02-19
AnnouncementXML	Submission_2026-02-19_10:44:22.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Muenzner
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	iodoacetamide derivatized residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 05:27:11	ID requested
⏵ 1	2026-02-19 10:44:23	announced

Dataset with its publication pending

submitter keyword: telomeres, evolution, genome instability,yeast, DNA damage response

Prof. Markus Ralser
contact affiliation	Charité Universitätsmedizin Berlin, Germany
contact email	markus.ralser@charite.de
lab head
Julia Muenzner
contact affiliation	Charite University Medicine, Berlin, Germany
contact email	julia.muenzner@charite.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064562

PRIDE project URI

• PRIDE
  1. PXD064562
     1. Label: PRIDE project
     2. Name: The adaptive molecular landscape to reprogrammed telomeric sequences