PXD064560

PXD064560 is an original dataset announced via ProteomeXchange.

Title	CacyBP/SIP - RPL6 interaction: potential influence on ribosome function
Description	Previously, we have shown that CacyBP/SIP interacts with NPM1, a protein involved in ribosome biogenesis. In this work, examined the potential impact of CacyBP/SIP on ribosome biogenesis and/or function. At first, we searched for ribosomal proteins (RPs) that might interact with CacyBP/SIP. Using mass spectrometry analysis, we have found that several RPs could be potential CacyBP/SIP targets. Then, by applying various biochemical methods, we confirmed that the interaction between CacyBP/SIP and RPL6 was direct. Moreover, in silico analysis allowed us to establish the domains/fragments of both proteins involved in this interaction. Subsequently, we performed several analyses using neuroblastoma NB2a cell line with stably silenced CacyBP/SIP expression. We have found, by applying OPP (O-propargyl-puromycin), that the number of cells with protein synthesis signal ("translation signal") was significantly lower in the cell line with diminished CacyBP/SIP level than in control one. Moreover, we observed lower induction of the synthesis of Hsp70, a stress-inducible protein, in NB2a cells with diminished CacyBP/SIP level after heat shock. In parallel, we have shown that CacyBP/SIP may have an impact on ER architecture/network. This confirms the influence of CacyBP/SIP, most probably through interaction with RPL6 and/or other RPs, on ribosome function and, in consequence, on protein synthesis in the cell.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:07:10.206.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064560
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Agata Malinowska
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	LTQ Orbitrap Velos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 04:30:41	ID requested
⏵ 1	2025-07-27 16:07:11	announced

10.1007/s00726-025-03464-3;

Jurewicz E, Maksymowicz-Trivedi M, Saberi-Khomami O, Iwa, ń, ska O, Starosta A, Kila, ń, czyk E, Bieganowski P, Jarmu, ł, a A, Le, ś, niak W, Filipek S, Filipek A, CacyBP/SIP - RPL6 interaction: potential influence on ribosome function. Amino Acids, 57(1):37(2025) [pubmed]

10.6019/PXD064560;

submitter keyword: mass spectrometry, IP,CacyBP/SIP

RPs

Ewelina Jurewicz
contact affiliation	Laboratory of Calcium Binding Protein, Nencki Institute of Experimental Biology PAS, Warsaw, Poland
contact email	e.jurewicz@nencki.edu.pl
lab head
Agata Malinowska
contact affiliation	IBB PAS
contact email	esme@ibb.waw.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064560
     1. Label: PRIDE project
     2. Name: CacyBP/SIP - RPL6 interaction: potential influence on ribosome function