PXD064536
PXD064536 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Evaluation of a modified Orbitrap Astral Zoom prototype for quantitative proteomics - Beyond identification lists |
Description | Mass spectrometry instrumentation continues to evolve rapidly, yet quantifying these advances beyond conventional peptide and protein detections remains challenging. Here, we evaluate a modified Orbitrap Astral Zoom mass spectrometer (MS) prototype and compare its performance to the standard Orbitrap Astral MS. Across a range of acquisition methods and sample inputs, the prototype instrument outperformed the standard Orbitrap Astral MS in precursor and protein identifications, ion accumulation efficiency, and reproducibility of measurements. To enable meaningful cross-platform comparisons, we implemented an ion calibration framework that converts signal intensity from arbitrary units to absolute ion counts (ions/sec). This benchmarking strategy showed that the prototype consistently sampled more ions per peptide, resulting in improved sensitivity and quantitative precision. To make these metrics broadly accessible, we added new metrics to the Skyline document grid to report ion counts directly from data-independent acquisition (DIA) data. Taken together, our results demonstrate the Orbitrap Astral Zoom prototype as a high-performance platform for DIA proteomics and establish a generalizable framework for ion-based evaluation of mass spectrometer performance. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2025-06-08 |
AnnouncementXML | Submission_2025-06-08_17:44:31.498.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Chris Hsu |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Gallus gallus; NCBI TaxID: 9031; |
ModificationList | Carbamidomethyl |
Instrument | Orbitrap Astral; Orbitrap Astral Zoom; Stellar; Orbitrap Exploris 480; Orbitrap Ascend; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-06-02 21:25:19 | ID requested | |
⏵ 1 | 2025-06-08 17:44:31 | announced |
Publication List
Hsu C, Shulman N, Stewart H, Petzoldt J, Pashkova A, Plubell D, Denisov E, Hagedorn B, Damoc E, MacLean BX, Remes P, Canterbury J, Makarov A, Hock C, Zabrouskov V, Wu CC, MacCoss MJ. Evaluation of a prototype Orbitrap Astral Zoom mass spectrometer for quantitative proteomics – Beyond identification lists. bioRxiv [Internet]. 2025 Jan 1;2025.05.30.657132. Available from: http://biorxiv.org/content/early/2025/06/02/2025.05.30.657132.abstract |
Keyword List
submitter keyword: ions, calibration, poisson statistics, astral, astral zoom, instrument comparison |
Contact List
Michael MacCoss | |
---|---|
contact affiliation | University of Washington |
contact email | maccoss@uw.edu |
lab head | |
Chris Hsu | |
contact affiliation | University of Washington |
contact email | chrhsu@uw.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |