PXD064520

PXD064520 is an original dataset announced via ProteomeXchange.

Title	Identification of binding partners of the purified TOM complex from HEK293T cells
Description	To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under native conditions. Our primary aim was to uncover human-specific interactors not previously described in yeast, with a particular focus on factors potentially involved in mitochondrial protein import regulation and quality control. The dataset provides a resource for understanding conserved and divergent aspects of TOM complex composition and function in human cells.
HostingRepository	PRIDE
AnnounceDate	2026-03-17
AnnouncementXML	Submission_2026-03-17_08:25:20.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-02 09:31:38	ID requested
⏵ 1	2026-03-17 08:25:21	announced

Dataset with its publication pending

submitter keyword: TOM complex, TOMM22-FLAG IP, HEK293T

Agnieszka Chacinska
contact affiliation	IMol Polish Academy of Sciences, Warsaw, Poland
contact email	a.chacinska@imol.institute
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064520

PRIDE project URI

• PRIDE
  1. PXD064520
     1. Label: PRIDE project
     2. Name: Identification of binding partners of the purified TOM complex from HEK293T cells