PXD064515

PXD064515 is an original dataset announced via ProteomeXchange.

Title	ChAHP Silences SINE Retrotransposons by Inhibiting TFIIIB Recruitment
Description	Short interspersed nuclear elements (SINEs) are abundant non-autonomous transposable elements derived from RNA polymerase III (Pol III)-transcribed short non-coding RNAs. SINEs retain sequence features recognized by the Pol III machinery and constitute a substantial portion of vertebrate genomes. Despite their impact on genome stability and evolution, the mechanisms governing SINE transcription remain poorly understood. Although DNA methylation and heterochromatin formation have been implicated in their repression, we find these pathways play only a minor role in mouse embryonic stem cells. Instead, we identify the ChAHP complex as a key repressor of SINE B2 elements. ChAHP directly inhibits Pol III transcription by blocking TFIIIB recruitment without affecting TFIIIC binding. This selective interference prevents transcription initiation and highlights a distinct regulatory mechanism. Our findings establish ChAHP as a non-canonical repressor of Pol III-dependent SINE transcription, offering new insights into the control of this pervasive class of non-coding genomic elements.
HostingRepository	PRIDE
AnnounceDate	2026-06-30
AnnouncementXML	Submission_2026-06-30_00:10:00.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Seebacher
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-02 07:32:03	ID requested
⏵ 1	2026-06-30 00:10:01	announced

10.1016/J.MOLCEL.2026.06.024;

submitter keyword: Immunoprecipitation-mass spectrometry (IP-MS), Label-free quantification, Proteomic validation, MaxQuant, iBAQ, Protein-protein interactions, einprot, Interactome analysis

Marc Buehler
contact affiliation	Genome Regulation, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
contact email	marc.buehler@fmi.ch
lab head
Jan Seebacher
contact affiliation	FMI Basel
contact email	jan.seebacher@fmi.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064515
     1. Label: PRIDE project
     2. Name: ChAHP Silences SINE Retrotransposons by Inhibiting TFIIIB Recruitment