PXD064482

PXD064482 is an original dataset announced via ProteomeXchange.

Title	Lead Exposure During Development Alters Synaptic Organization and Protein Expression in Rat Hippocampus
Description	Lead (Pb) is a potent neurotoxin with disastrous effects on cognition, memory, and sensory functions. Pb exposure during the developmental stage of life causes irreversible changes in neuronal morphology impacting the physiological functions from reduced motor skills and lowered intelligence quotient to developmental disorders and delayed puberty. To investigate the effect of developmental Pb exposure on the rodent hippocampus, we exposed rat pups to 100 ppm lead acetate via lactation and looked into the morphological and proteomic alterations in the hippocampal neurons. Our Nissl stain data revealed a significant reduction in the number of CA1 neurons in the hippocampus of the Pb-exposed rats. Further, we analyzed the dendritic tree morphology of the CA1 neurons and reported neuronal hypertrophy and a reduction in spine density in both the basal and the apical dendrites in Pb-exposed rats. Our mass spectrometric data identified a total of 6780 proteins out of which 190 proteins were p-value significant. We reported a total of 31 dysregulated proteins in the hippocampus of Pb-exposed rats. The changes seen in the protein expression profile and reduction in neuronal numbers in addition to the altered dendritic tree of Pb-exposed neurons suggest the molecular and morphological changes that could underlie the memory and cognitive deficits seen in Pb-exposed animals.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_18:22:07.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bhupesh Mehta
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-31 16:57:10	ID requested
⏵ 1	2025-11-30 18:22:08	announced

10.1016/j.jhazmat.2025.140582;

Shirke O, Velu S, Pandita S, Saha AK, Patil RR, Joshi NS, Govindaraj P, Rao LT, Markandeya YS, Mehta B, Lead exposure during development alters synaptic organization and protein expression in rat hippocampus. J Hazard Mater, 500():140582(2025) [pubmed]

submitter keyword: proteomics, hippocampus, histology, memory,Pb toxicity

Bhupesh Mehta
contact affiliation	Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS). India.
contact email	bhupeshmehta@nimhans.ac.in
lab head
Bhupesh Mehta
contact affiliation	National Institute of Mental Health and Neurosciences
contact email	bhupeshmehta@nimhans.ac.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD064482

PRIDE project URI

• PRIDE
  1. PXD064482
     1. Label: PRIDE project
     2. Name: Lead Exposure During Development Alters Synaptic Organization and Protein Expression in Rat Hippocampus