PXD064480

PXD064480 is an original dataset announced via ProteomeXchange.

Title	Changes in Potential Pathogenicity-Associated Proteins of Helicobacter cinaedi Upon Infection of Macrophage Cells
Description	Helicobacter cinaedi is a spiral-shaped Gram-negative, enterohepatic bacterium classified as a conditional pathogen (pathogenicity group 2). It is known to cause bacteremia and a variety of other diseases in humans. Notably, H. cinaedi has been shown to induce changes in cell morphology, including the transition to “foam cells”, when interacting with macrophages. To uncover protein factors involved in the pathogenesis of Helicobacter cinaedi, we performed a detailed mass spectrometric analysis of the proteome of strain BAA-847. To the best of our knowledge, this is the first such comprehensive study examining the bacterium's protein profile both under standard culture conditions and after infection of M1-type macrophage cells. We identified 1,575 proteins in the H. cinaedi proteome, and of these, 109 proteins were differentially upregulated after infection of macrophage cells. For these proteins, we performed a detailed functional analysis and proposed a model to explain their potential roles during Helicobacter cinaedi infection. In addition to the known pathogenicity factors HcaA, Cdt, and AhpC, we discovered several poorly understood proteins that are involved in immune response evasion, adaptation for survival within host cells, and possess toxic or other potentially pathogenic functions. Among the differentially upregulated proteins, we found those that enable the bacterium to utilize intracellular cholesterol as a carbon source. Additionally, we identified proteins involved in a toxin injection mechanism that disrupts host cell metabolism, which may be linked to the foam cell formation induced by Helicobacter cinaedi.
HostingRepository	PRIDE
AnnounceDate	2025-06-01
AnnouncementXML	Submission_2025-06-01_01:03:55.592.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064480
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Georgij Arapidi
SpeciesList	scientific name: Helicobacter cinaedi CCUG 18818 = ATCC BAA-847; NCBI TaxID: 537971;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-31 16:34:03	ID requested
⏵ 1	2025-06-01 01:03:55	announced

10.6019/PXD064480;

submitter keyword: bacterial infection of eukaryotic cells, pathogen escape from cellular immunity, mass-spectrometry, bacterial pathogenicity, macrophages, proteome analysis,Helicobacter cinaedi, virulence factor

Georgij Arapidi
contact affiliation	System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Russian Federation
contact email	arapidi@gmail.com
lab head
Georgij Arapidi
contact affiliation	Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
contact email	arapidi@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064480
     1. Label: PRIDE project
     2. Name: Changes in Potential Pathogenicity-Associated Proteins of Helicobacter cinaedi Upon Infection of Macrophage Cells