PXD064478

PXD064478 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Six Protein Mix (Pierce) System Suitability Experiments
Description	This repository entry contains a set of fifty-eight (58) separate LC-MS/MS injections of the Pierce 6 Protein Digest, equimolar, LC-MS grade (Thermo PN: 88342). These data were acquired over a duration of one month to assess system suitability of a nanoLC-MS/MS system. Here an Ultimate nanoRSLC system in a "tandem pre-concentration" setup was coupled the nanoFlex source on an Orbitrap Fusion Lumos mass spectrometer. Peak lists were extracted from mass spectrometry data using ThermoRawFileParser. Mascot Server was used to compare tandem mass spectra to the reference sequences provided in this submission. Protein identification data are provided in both DAT and mzID formats. Proteome informatics are described in detail in the following publications from our group: Bird LJ et al, ACS Synthetic Biology, 2023; Coppens L et al. Molecular Systems Biology, 2023.
HostingRepository	MassIVE
AnnounceDate	2025-05-31
AnnouncementXML	Submission_2025-05-31_13:42:25.036.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	J Hervey
SpeciesList	scientific name: standard protein mixture;
ModificationList	iodoacetamide - site C; unknown modification
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-31 13:02:56	ID requested
⏵ 1	2025-05-31 13:42:25	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Protein Standard, System Suitability, Six Protein Digest, equimolar (Pierce/Thermo), nanoLC, Orbitrap, nanoLC-MS/MS, DatasetType:Proteomics

submitter keyword: Protein Standard, System Suitability, Six Protein Digest, equimolar (Pierce/Thermo), nanoLC, Orbitrap, nanoLC-MS/MS, DatasetType:Proteomics

Contact List

J Hervey
contact affiliation	NRL
contact email	judson.hervey@nrl.navy.mil
lab head
J Hervey
contact affiliation	NRL
contact email	judson.hervey@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098062/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098062/