PXD064454

PXD064454 is an original dataset announced via ProteomeXchange.

Title	A not so minor role for the minor spliceosome in spermatogenesis
Description	Minor introns have been highly conserved in the genome since their emergence in the last eukaryotic common ancestor and are found in genes related to proliferation, chromatin organization, transcription, and splicing. Here, we show that minor intron-containing genes (MIGs) involved in the gene expression landscape of mouse spermatogenesis are largely conserved in chordates as MIGs, which rely on the minor spliceosome (MiS) and its small nuclear RNAs (snRNAs) for their minor intron splicing. We show that MIGs are highly enriched in regulating, mitosis, meiosis and specifically sperm differentiation. Stra8-Cre mediated ablation of U11 snRNA inhibited the minor spliceosome in differentiating spermatogonia and spermatocytes, which resulted in both mitosis and meiosis defects that resulted in severe reduction of mature sperm in the seminiferous tubule leading to testicular defect. Increased death of spermatocytes was observed in mutants due to defective meiotic chromosome features including DNA damage repair, telomere morphology, synapsis of homologous chromosomes and XY-chromosome association. These phenotypes were underscored by the aberrant splicing and differential expression of core spermatogenesis genes, including Hfm1. In all, we position spermatogenesis as another selective pressure that ensured conservation and expansion of MIGs in the genome.
HostingRepository	PRIDE
AnnounceDate	2026-03-04
AnnouncementXML	Submission_2026-03-03_16:02:29.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeremy Balsbaugh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-30 09:06:40	ID requested
⏵ 1	2026-03-03 16:02:29	announced

10.1093/nar/gkag159;

Takemoto K, Girardini KN, Boria A, Rosado J, Konakanchi T, Vachhani S, Springer S, Kanadia RN, Minor spliceosome inhibition via ablation of its U11 snRNA gene results in multiple defects in murine spermatogenesis. Nucleic Acids Res, 54(5):(2026) [pubmed]

submitter keyword: timsTOF HT, data independent acquisition,spliceosome, spermatogenesis

Dr. Jeremy Balsbaugh
contact affiliation	Dr. Jeremy Balsbaugh, Proteomics & Metabolomics Facility, Center for Open Research Resources & Equipment University of Connecticut Storrs, CT 06269
contact email	jeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliation	University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064454

PRIDE project URI

• PRIDE
  1. PXD064454
     1. Label: PRIDE project
     2. Name: A not so minor role for the minor spliceosome in spermatogenesis