PXD064452 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | hnRNPR regulates alternative splicing in an m6A-dependent manner required for spermiogenesis and male fertility in humans and mice |
Description | Male infertility is an increasingly pressing global health concern, with abnormal spermiogenesis being a major cause of asthenoteratozoospermia. Although RNA-binding proteins (RBPs)-orchestrated alternative splicing has recently garnered significant attention, its regulation mechanisms during spermiogenesis remain unclear. Here, we identify hnRNPR as a crucial m6A-dependent splicing regulator. Mutations in hnRNPR lead to asthenoteratozoospermia in both humans and mice. Mechanistically, loss of hnRNPR exhibits widespread transcriptomic, proteomics, and m6A-modified splicing dysregulation, disrupting these genes essential for acrosome formation and flagellum development. Remarkably, Skap2 knockdown mice exhibit sperm defects that phenocopy those seen in Hnrnpr mutants. Importantly, therapeutic delivery of SKAP2, via extracellular vesicles, restores F-actin-mediated cytoskeletal integrity and improves sperm motility. Collectively, these findings establish that spermiogenesis is controlled post-transcriptionally by splicing in an m6A-dependent manner and reveal a promising therapeutic contribution for male infertility. |
HostingRepository | PRIDE |
AnnounceDate | 2025-06-04 |
AnnouncementXML | Submission_2025-06-03_17:13:28.923.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | 世明 甘 |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-05-30 08:28:52 | ID requested | |
⏵ 1 | 2025-06-03 17:13:29 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
Contact List
gan shiming |
contact affiliation | zhejiang university |
contact email | 1206682912@qq.com |
lab head | |
世明 甘 |
contact affiliation | tongji |
contact email | 1206682912@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064452
- Label: PRIDE project
- Name: hnRNPR regulates alternative splicing in an m6A-dependent manner required for spermiogenesis and male fertility in humans and mice