PXD064389 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16 |
| Description | Cyclin-dependent protein kinase 16 (CDK16) regulates both physiological and pathological processes, including autophagy, spermatogenesis and cancer. Unlike other CDKs, CDK16 is regulated by an unclear mechanism involving phosphorylated cyclin Y (CCNY) in complex with 14-3-3 proteins rather than CCNY alone. The present study aims at elucidating this mechanism by structurally characterizing CDK16 in complex with CCNY and 14-3-3 using several biophysical techniques. As shown by cryo-EM analysis and hydrogen/deuterium exchange coupled to mass spectrometry, 14-3-3 binding unmasks a key moiety of the CDK binding surface of CCNY, thereby enabling CDK16 activation. CDK16 interacts with the cyclin box of CCNY, while 14-3-3 provides additional contacts, including with the activation segment of CDK16. CDK16 activation also requires interactions of CCNY with the N-terminal extension of CDK16. These findings not only clarify the role of CCNY and 14-3-3 in CDK16 activation but also highlight the potential of targeting CDK16 protein-protein interactions for cancer therapy. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-25 |
| AnnouncementXML | Submission_2026-03-25_00:52:34.746.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Pavla Vankova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-28 17:46:49 | ID requested | |
| ⏵ 1 | 2026-03-25 00:52:35 | announced | |
Publication List
Keyword List
| submitter keyword: CDK16,Cyclin-dependent protein kinase, CCNY, cyclin Y, 14-3-3 protein |
Contact List
| Tomas Obsil |
|---|
| contact affiliation | Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic Institute of Physiology of the Czech Academy of Sciences, Laboratory of Structural Biology of Signaling Proteins, Division BIOCEV, Vestec, Czech Republic |
| contact email | tomas.obsil@natur.cuni.cz |
| lab head | |
| Pavla Vankova |
|---|
| contact affiliation | Institute of Biotechnology of the Czech Academy of Sciences, BioCeV, Prumyslova 595, 252 50 Vestec, Czech Republic |
| contact email | pavla.vankova@ibt.cas.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064389 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064389
- Label: PRIDE project
- Name: Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16
