PXD064376

PXD064376 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Establishing a MS-based assay for in vivo peptide deformylase inhibition in Bacillus subtilis
Description	The peptide deformylase inhibitor actinonin was used to treat Bacillus subtilis 168, and the proteome was analyzed using UPLC-MSE. The goal was to identify proteins that retain N-terminal formylation of the initial methionine after actinonin treatment that could be used to establish a microplate assay based on mass spectrometry for in vivo peptide deformylase inhibition.
HostingRepository	PRIDE
AnnounceDate	2026-02-11
AnnouncementXML	Submission_2026-02-11_06:27:20.891.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sina Schäkermann
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: NEWT:224308;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-28 07:06:38	ID requested
⏵ 1	2026-02-11 06:27:21	announced

Publication List

10.1016/J.EJMECH.2026.118678;

10.1016/J.EJMECH.2026.118678;

Keyword List

submitter keyword: peptide deformylase, antibiotics,proteomics, N-formylation, Bacillus subtilis

submitter keyword: peptide deformylase, antibiotics,proteomics, N-formylation, Bacillus subtilis

Contact List

Julia E. Bandow
contact affiliation	Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, Universitätsstraße 150, Bochum 44801, Germany
contact email	julia.bandow@rub.de
lab head
Sina Schäkermann
contact affiliation	Ruhr-Universität Bochum
contact email	sina.langklotz@rub.de
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064376
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD064376

PRIDE project URI

Repository Record List

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