PXD064338

PXD064338 is an original dataset announced via ProteomeXchange.

Title	Mitochondria drive a folate-based immunity against an intracellular pathogen
Description	As major consumers of cellular metabolites, mitochondria are poised to compete with invading microbes for the nutrients they need to grow. Yet, whether cells weaponize mitochondrial metabolism during infection remains poorly understood. We found that the transcription factor ATF4 activates a mitochondrial defense based on the essential B vitamin folate. During infection with the intracellular pathogen Toxoplasma gondii, ATF4 increased mitochondrial DNA levels by driving the one-carbon metabolism processes that use folate in mitochondria. Triggered by host detection of mitochondrial stress induced by parasite effectors, ATF4 limited Toxoplasma access to folates required for dTMP synthesis, thereby restricting parasite growth. Thus, ATF4 rewires mitochondrial metabolism to mount a folate-based metabolic immunity against Toxoplasma.
HostingRepository	PRIDE
AnnounceDate	2025-10-02
AnnouncementXML	Submission_2025-10-02_04:36:50.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-27 09:27:27	ID requested
⏵ 1	2025-10-02 04:36:51	announced

10.1126/science.adr6326;

Medeiros TC, Ovciarikova J, Li X, Krueger P, Bartsch T, Reato S, Crow JC, Tellez Sutterlin M, Martins Garcia B, Rais I, Allmeroth K, Hartman MD, Denzel MS, Purrio M, Mesaros A, Leung KY, Greene NDE, Sheiner L, Giavalisco P, Pernas L, Mitochondria protect against an intracellular pathogen by restricting access to folate. Science, 389(6761):eadr6326(2025) [pubmed]

submitter keyword: mitochondria,TMT

Lena Pernas
contact affiliation	UCLA, Charles E. Young Drive CHS 26-100 Los Angeles, CA 90095
contact email	lfpernas@mednet.ucla.edu
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	ilian.atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD064338

PRIDE project URI

• PRIDE
  1. PXD064338
     1. Label: PRIDE project
     2. Name: Mitochondria drive a folate-based immunity against an intracellular pathogen