PXD064299 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Untargeted NoPro-clipping from untramsformed and Hoil-1L over-expressing Huh7 cell lines |
| Description | NoPro-clipping is a method for detecting and quantifying non-proteinaceous ubiquitination from cell lysates and mammalian tissue. The method utilising Ub-clippases to cleave ubiquitin but leave the C-terminal diglycine remnant attached to the protein and non-protein substrates. Following protein exclusion, small molecule diglycine species are selectively labelled with an LPXTGG-containing peptide via the enzyme sortase. While the method can be employed in a targeted workflow to quantify a pre-defined non-protein ubiquitin species, the method can also be employed in an untargeted approach. This submission includes the necessary controls and raw files to identify non-protienaceous ubiquitination present in Huh7 cells either untransformed or over-expressing the E3 ligase HOIL-1L fused to a di-ubiquitin (for increased activity). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-29 |
| AnnouncementXML | Submission_2026-07-28_20:46:13.663.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Simon Cobbold |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-05-26 03:42:54 | ID requested | |
| ⏵ 1 | 2026-07-28 20:46:14 | announced | |
Publication List
Keyword List
| submitter keyword: NoPro clipping, Ubiquitin, untargeted, Non-protein |
Contact List
| Simon Cobbold |
|---|
| contact affiliation | Ubiquitin Signalling Division WEHI |
| contact email | cobbold.s@wehi.edu.au |
| lab head | |
| Simon Cobbold |
|---|
| contact affiliation | WEHI |
| contact email | cobbold.s@wehi.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD064299 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064299
- Label: PRIDE project
- Name: Untargeted NoPro-clipping from untramsformed and Hoil-1L over-expressing Huh7 cell lines
