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PXD064272

PXD064272 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExploring the Cytotoxic Mechanisms and Anticancer Potentials of Silver Nanoparticles in Cisplatin-Sensitive and -Resistant Lung Adenocarcinoma: Insights from Proteomic and Mice Xenograft Studies
DescriptionSilver nanoparticles (AgNPs) have shown great potential as therapeutic agents due to their ability to cause apoptotic cell death in cancer cells. However, little knowledge is available regarding the underlying action mechanisms of AgNPs towards multi-drug resistant cancer cells. Herein, we employed quantitative proteomics to investigate the cytotoxic mechanisms of AgNPs on both cisplatin-sensitive (A549 cells) and -resistant (A549/DDP cells) human lung adenocarcinoma and to explore their potential anticancer abilities. We first performed cytotoxicity tests and found that AgNPs exert similar cytotoxic effects on A549 and A549/DDP cells. At the proteome level, A549 and A549/DDP cells responded to AgNPs distinctively and similarly by causing cell apoptosis via upregulating RNA metabolism, suppressing VEGF siganling pathway, repressing p53-mediated pathways, promoting cell cycle arrest, etc. Additionally, AgNPs remarkably induced ROS generation in A549 and A549/DDP cells. The mitotoxicity results further confirmed the effectiveness of AgNPs in hampering mitochondrial function and respiration in A549 and A549/DDP cells. Overall, our investigations showed that AgNPs could effectively induce cell deaths in human lung adenocarcinoma cells regardless of their sensitivities to cisplatin, suggesting that AgNPs could be potentially used in biomedical aspects as an anticancer agent in alleviating the problem of acquired drug resistance in chemotherapy.
HostingRepositoryPRIDE
AnnounceDate2025-05-30
AnnouncementXMLSubmission_2025-05-30_09:08:49.553.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTin Yan Wong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-25 05:51:58ID requested
12025-05-30 09:08:49announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Silver nanoparticles, Resistance,Proteomics, NSCLC, Cisplatin
Contact List
Henry Lam
contact affiliationThe Hong Kong University of Science and Technology
contact emailkehlam@ust.hk
lab head
Tin Yan Wong
contact affiliationThe Hong Kong University of Science and Technology
contact emailtywongam@connect.ust.hk
dataset submitter
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Dataset FTP location
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