PXD064240

PXD064240 is an original dataset announced via ProteomeXchange.

Title	TUG protein acts through a disordered region to organize the early secretory pathway
Description	The Endoplasmic Reticulum (ER)-Golgi Intermediate Compartment (ERGIC) is a network of tubules and vesicles known for producing COPI vesicles and receiving COPII vesicles from the ER. Much about its identity, stability, and regulation remains unknown. Here, we show that the TUG (UBXN9, Aspscr1) protein, a central regulator of GLUT4 trafficking, localizes to the ERGIC, and its deletion enhances anterograde secretory flux, redistributes ERGIC markers to the cis-Golgi, and alters Golgi morphology. TUG forms biomolecular condensates in vitro and contains a central disordered region that mediates its recruitment to ERGIC membranes. A distinct N-terminal region mediates its oligomerization in cells. TUG deletion disrupts ERGIC-dependent processes, including autophagy and collagen secretion, and alters the targeting of CFTR, a model protein that undergoes unconventional secretion. We conclude that TUG organizes and stabilizes ERGIC membranes to support their roles in diverse cellular membrane trafficking pathways.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_08:27:03.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 07:22:07	ID requested
⏵ 1	2025-06-10 08:27:04	announced

10.1038/S41467-025-60691-8;

submitter keyword: Golgi, Aspscr1, Biomolecular condensates,Membrane trafficking, ERGIC, TUG

Ishier RAOTE
contact affiliation	Université Paris Cité/CNRS, Institut Jacques Monod, FRANCE
contact email	ishier.raote@ijm.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064240
     1. Label: PRIDE project
     2. Name: TUG protein acts through a disordered region to organize the early secretory pathway