PXD064235

PXD064235 is an original dataset announced via ProteomeXchange.

Title	Unravel cellular heterogeneity in IDH1-mutant low-grade glioma cell Lines
Description	IDH1-mutant low-grade glioma are slow growing brain tumours arising from glial cells. They are subdivided in two groups; oligodendrogliomas and astrocytomas. They are characterizing by an intra-tumoral cellular heterogeneity. Previous studies have shown that at least 3 populations can be found in these tumours; astrocyte-like cells, oligodendrocyte-like cells and stem-like cells. Among these populations, some cells seem to be in a quiescent-like state, where others are mitotic. However, the correlation between glioma cell phenotype and cell cycle is still not fully explored. Here, using a low-grade glioma cell line named LGG275 published in Cancers 2021, 13(9), 2107; https://doi.org/10.3390/cancers13092107, we provided a method to sort at least two populations; the astrocyte-like cells and the oligodendrocyte-like cells, based on two extracellular markers, in a patient derived astrocytoma cell line. Proteomic analysis of these two populations as revealed that one is associated to a quiescent state, is plastic and can drive cellular heterogeneity, where the other is associated to an active state, and has a role in stem cell maintenance.
HostingRepository	PRIDE
AnnounceDate	2026-07-22
AnnouncementXML	Submission_2026-07-22_06:13:43.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 05:46:34	ID requested
⏵ 1	2026-07-22 06:13:44	announced

10.1186/S40478-026-02316-9;

submitter keyword: Human, quiescence, cell Lines, glioma cells, cellular heterogeneity, low-grade glioma

Jean-Philippe Hugnot
contact affiliation	Institute of Functional Genomics, Univ. Montpellier, CNRS, INSERM, Montpellier, France
contact email	jean-philippe.hugnot@umontpellier.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD064235

PRIDE project URI

• PRIDE
  1. PXD064235
     1. Label: PRIDE project
     2. Name: Unravel cellular heterogeneity in IDH1-mutant low-grade glioma cell Lines