PXD064233

PXD064233 is an original dataset announced via ProteomeXchange.

Title	The mass spectrometric intact transition epitope mapping method supports protein engineering of foldon trimer variants
Description	The wild-type foldon (foldon 0) is the C-terminal trimerization domain of the bacteriophage T4 Fibritin foldon (T4Ff). T4Ff’s amino acid sequence is GYIPEAPRDGQAYVRKDGEWVLLSTFL (27 amino acid residues; single letter code). Foldons 0, 1-6 were synthesized by solid phase peptide synthesis and in foldons 1-6 D amino acids at positions 10 (G10a) and 17 (D17f) were introduced. In addition, foldons 2-6 carry artificial N-termini consisting of amino acid residues with increasing space-demanding side chains. All foldons, 0-6, form non-covalent trimers in solution and courses of trimer dissociations were investigated after electrospraying supramolecular ions in the gas phase using ITEM-FIVE (Intact Transition Epitope Mapping - Force Interferences by Variable Extensions) mass spectrometry. Differences in trimer stabilities were correlated with structural features.
HostingRepository	PRIDE
AnnounceDate	2025-11-28
AnnouncementXML	Submission_2025-11-27_22:58:14.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Enterobacteria phage T4 (Bacteriophage T4); NCBI TaxID: NEWT:10665;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-23 04:20:04	ID requested
⏵ 1	2025-11-27 22:58:15	announced

10.1038/S41598-025-28101-7;

submitter keyword: None

Michael O.
contact affiliation	Director, Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@med.uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD064233

PRIDE project URI

• PRIDE
  1. PXD064233
     1. Label: PRIDE project
     2. Name: The mass spectrometric intact transition epitope mapping method supports protein engineering of foldon trimer variants