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PXD064223

PXD064223 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of SOX2 associated protein complexes in mouse ES cells
DescriptionTo uncover novel components of the SOX2-associated protein complex, we established E14 mouse embryonic stem (ES) cells stably expressing Flag-HA-tagged SOX2. Whole-cell extracts were prepared and subjected to tandem affinity purification, sequentially using anti-Flag M2 and anti-HA agarose resins . The purified SOX2 complex was separated by SDS-PAGE and visualized by silver staining . Distinct protein bands specifically enriched in the SOX2 immunoprecipitate were excised and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) .
HostingRepositoryjPOST
AnnounceDate2026-05-23
AnnouncementXMLSubmission_2026-05-22_08:00:04.698.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNaoko Hattori
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
InstrumentLTQ Orbitrap Velos Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-22 23:27:11ID requested
12026-05-22 08:00:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SOX2, TBP, ES cell
Contact List
Takeya Nakagawa
lab head
Naoko Hattori
contact affiliationNagasaki University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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