PXD064221

PXD064221 is an original dataset announced via ProteomeXchange.

Title	The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation
Description	BRD4 regulates gene transcription in complex eukaryotes, in part through the binding of its tandem bromodomains to acetylated lysine residues found in histones and transcription factors. Despite pharmacological inhibition of these binding events showing significant promise in preclinical studies, clinical trial data have been less encouraging so far. A stronger understanding of BRD4 biochemistry could provide a route to better outcomes. To advance on prior work, which has focused almost entirely on the binding properties of isolated bromodomains and acetylated peptides, we have sought the preferred nucleosomal binding partner of full-length BRD4. Here we performed affinity pulldowns of HEK293 mononucleosomes against BRD4 to ascertain binding preferences in the nucleosomal context.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_16:31:24.514.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Low
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	propanoylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 23:18:55	ID requested
⏵ 1	2026-01-11 16:31:25	announced

10.1093/nar/gkaf1406;

Lambrechts LS, Reid XJ, Kambanis L, Luong C, Kobakhidze E, Daners A, Zhong Y, Patel K, Franck CK, Sani HM, Taylor C, Low JKK, Payne RJ, Mackay JP, The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation. Nucleic Acids Res, 53(22):(2025) [pubmed]

submitter keyword: histone, nucleosome, PTMs, acetylation,BRD4

Joel Mackay
contact affiliation	School of Life and Environmental Sciences, University of Sydney, NSW 2006 Australia
contact email	joel.mackay@sydney.edu.au
lab head
Jason Low
contact affiliation	The University of Sydney
contact email	jason.low@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD064221

PRIDE project URI

• PRIDE
  1. PXD064221
     1. Label: PRIDE project
     2. Name: The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation