PXD064208

PXD064208 is an original dataset announced via ProteomeXchange.

Title	Proteomics approach to identify sample manipulation in forensic toxicology for hair and urine
Description	Urine and hair are among the primary biological matrices used for drug and abstinence testing in clinical, forensic, and anti-doping settings. A persistent challenge in such analyses is the adulteration of samples: through dilution, substitution, or chemical modification, aimed at concealing the presence of xenobiotics such as drugs of abuse, ethanol, or doping agents. In this study, we investigated whether chemical adulteration, specifically oxidative treatment with hydrogen peroxide (H2O2), induces detectable and characteristic changes in the proteomes of urine and hair samples. Using a bottom-up proteomics approach involving LC-HR-MS/MS with data-dependent acquisition, we compared urine samples before and after treatment (10% H2O2) and untreated hair samples with those exposed to increasing concentrations of hydrogen peroxide (3%, 6%, 9% and 12%). We identified distinct peptides, including oxidatively modified forms, that were exclusively present either in untreated or chemically treated groups. In hair samples, the appearance of some of those peptides was dependent on peroxide concentration. Peptides detectable only after oxidative exposure were of particular interest, as they appeared to be non-physiological and specific to the adulteration process. These species serve as candidate biomarkers for indirect detection of sample manipulation or for assessing the integrity of compromised samples. The extraction and characterization of these potential marker peptides constitute the primary outcome of this study. These findings should lay the groundwork for further validation and the development of proteomic methods aimed at enhancing the reliability of drug testing and sample authenticity assessment in forensic and anti-doping contexts.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_16:40:08.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Steuer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 13:32:03	ID requested
⏵ 1	2025-08-24 16:40:09	announced

10.1021/acs.analchem.5c03096;

Schneider TD, Binz TM, Kraemer T, Steuer AE, New Approaches To Identify Urine and Hair Adulteration Attempts in Forensic Toxicology: A Proof-of-Concept Study Using a Proteomics Approach Based on Liquid Chromatography-Mass Spectrometry (LC-MS). Anal Chem, 97(32):17761-17769(2025) [pubmed]

submitter keyword: forensics, hair, urine, LC-MS,manipulation

Andrea E. Steuer
contact affiliation	Zurich Institute of Forensic Medicine University of Zurich Switzerland
contact email	andrea.steuer@irm.uzh.ch
lab head
Andrea Steuer
contact affiliation	Zurich Institute of Forensic Medicine University of Zurich
contact email	andrea.steuer@irm.uzh.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD064208

PRIDE project URI

• PRIDE
  1. PXD064208
     1. Label: PRIDE project
     2. Name: Proteomics approach to identify sample manipulation in forensic toxicology for hair and urine