PXD064200

PXD064200 is an original dataset announced via ProteomeXchange.

Title	Non-lytic and active emission of cellular fragments carrying whole genome DNA during cancer cell migration
Description	Extracellular vesicles (EVs) are emerging as critical mediators of intercellular communication, carrying bioactive cargoes such as DNA, RNA, and proteins. However, the molecular mechanisms underlying the packaging and release of DNA within these EVs remain unclear. In this study, we identify a distinct class of large, non-lytic cellular fragments, termed Motile Vesicles (MoVes), emitted during active migration of cancer cells. Interestingly, non-transformed epithelial cells do not emit MoVes. We identified MoVes carry double-stranded replicative genomic DNA, histones, and mitochondrial proteins, and exhibit unique proteomic signatures enriched in integrins (ITGA2, ITGA3, ITGB1), Basigin (BSG), and the epithelial-to-mesenchymal transition (EMT) effector FAM3C. Whole-genome sequencing revealed that MoVes DNA mirrors the complete mutational landscape of the parent cancer cells. Notably, migration-inhibitory drugs significantly reduced the biogenesis of MoVes, while the neutral sphingomyelinase 2 (nSMAse2: sEV biogenesis pathway) inhibitor GW4869 had no effect. Moreover, inhibition of TGFβR1 using LY-364947 suppressed cell migration, ultimately preventing DNA emission via MoVes, linking MoVes formation to migration and EMT. In vivo biodistribution of MoVes were most abundant in the liver. Our findings uncover an active, non-apoptotic mechanism for genome-wide DNA release during cancer cells migration, with potential implications for metastasis and extracellular DNA biology.
HostingRepository	PRIDE
AnnounceDate	2026-08-28
AnnouncementXML	Submission_2026-08-28_15:12:59.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thupten Tsering
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 10:28:43	ID requested
⏵ 1	2026-08-28 15:13:00	announced

Dataset with its publication pending

submitter keyword: epithelial to mesenchymal transition, extracellular vesicles, EV associated DNA, migration,Motile Vesicle

Julia Valdemarin Burnier
contact affiliation	Assistant Professor, Departments of Oncology & Pathology, McGill University Principal Investigator, Cancer Research Program, MUHC-RI 1001 Decarie Blvd, EM2.2218 514.934.1934 ext 76307 Montréal, Canada
contact email	julia.burnier@mcgill.ca
lab head
Thupten Tsering
contact affiliation	RI-MUHC
contact email	thupten.tsering@mail.mcgill.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD064200

PRIDE project URI

• PRIDE
  1. PXD064200
     1. Label: PRIDE project
     2. Name: Non-lytic and active emission of cellular fragments carrying whole genome DNA during cancer cell migration