PXD064199

PXD064199 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	: Identification of interactors of Arabidopsis VIN3 wildtype and VIN3 chimeric mutant protein in vernalized seedlings
Description	The Arabidopsis VEL proteins VIN3 and VRN5 are accessory proteins of the Polycomb Repressive Complex 2 (PRC2). Their function is most well-understood in the epigenetic PRC2-mediated silencing of the floral repressor gene Flowering Locus C (FLC) during prolonged cold (vernalization). To identify protein interactors of VIN3 and VIN3 chimeric mutant protein carrying VRN5VEL instead of VIN3VEL, we used stable Arabidopsis transgenic lines expressing VIN3-GFP WT and VIN3-GFP VRN5VEL under endogenous promoters to perform native co-immunoprecipitation assays in seedlings vernalized for six weeks at 5 °C. Non-transgenic Col-FRI was used as a negative control.
HostingRepository	PRIDE
AnnounceDate	2025-08-28
AnnouncementXML	Submission_2025-08-28_03:52:10.470.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064199
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 10:28:25	ID requested
⏵ 1	2025-08-28 03:52:11	announced

Publication List

10.6019/PXD064199;
10.1016/j.molcel.2025.08.002;
Schulten A, Jang GJ, Payne-Dwyer A, Fiedler M, Nielsen ML, Mateo-Bonmat, í E, Bienz M, Leake MC, Dean C, VEL-dependent polymerization maintains the chromatin association of Polycomb proteins for the switch to epigenetic silencing. Mol Cell, 85(17):3321-3332.e5(2025) [pubmed]

10.6019/PXD064199;

10.1016/j.molcel.2025.08.002;

Schulten A, Jang GJ, Payne-Dwyer A, Fiedler M, Nielsen ML, Mateo-Bonmat, í E, Bienz M, Leake MC, Dean C, VEL-dependent polymerization maintains the chromatin association of Polycomb proteins for the switch to epigenetic silencing. Mol Cell, 85(17):3321-3332.e5(2025) [pubmed]

Keyword List

submitter keyword: PRC2, gene silencing, vernalization,epigenetics, Polycomb, VEL domain

submitter keyword: PRC2, gene silencing, vernalization,epigenetics, Polycomb, VEL domain

Contact List

Caroline Dean
contact affiliation	Research Professor Cell and Developmental Biology John Innes Centre Norwich Research Park Norwich, UK
contact email	caroline.dean@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD064199

PRIDE project URI

Repository Record List

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