PXD064197

PXD064197 is an original dataset announced via ProteomeXchange.

Title	The supernatant of the culture medium of adipocytes LC-MSMS
Description	Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease without effective medical therapies. Emerging evidences have suggested a crosstalk between adipose tissue and vascular cells and brown adipose tissue is beneficial for cardiovascular health. Nevertheless, whether brown remodeling of white adipose tissue would protect against AAA remains unclear. Here we showed that patients with AAA had a decreased browning level of adipose tissue and induction of adipose tissue browning significantly reduced AAA incidence and attenuated AAA development in mice. Using LC-MS/MS and proteomic analysis, we further identified Follistatin-like 1 (FSTL1) as a novel vessel-protective adipokine secreted by browning adipocytes. Mechanistically, FSTL1 inhibited VSMC apoptosis through DIP2A/AKT signaling. Furthermore, we demonstrated that adipocyte-specific deficiency of FSTL1 abrogated the protective effect of browning induction. Moreover, supplementation of FSTL1 either systemically or patched into hydrogel placing around abdominal aorta markedly limited aortic dilation and AAA progression. Our data suggest a protective role of adipose tissue browning and a novel batokine FSTL1 in the development of AAA, which may represent a novel intervention strategy for AAA.
HostingRepository	PRIDE
AnnounceDate	2025-09-07
AnnouncementXML	Submission_2025-09-07_05:13:45.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Yao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-22 09:50:17	ID requested
⏵ 1	2025-09-07 05:13:45	announced

Dataset with its publication pending

submitter keyword: Mus musculus,Supernatant,Adipocytes,LC-MSMS

Zhaoyu Liu
contact affiliation	Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China
contact email	liuzhy98@mail.sysu.edu.cn
lab head
Lei Yao
contact affiliation	Sun Yat-sen Memorial Hospital, Sun Yat-sen University
contact email	yaolei5@mail3.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064197

PRIDE project URI

• PRIDE
  1. PXD064197
     1. Label: PRIDE project
     2. Name: The supernatant of the culture medium of adipocytes LC-MSMS