PXD064170

PXD064170 is an original dataset announced via ProteomeXchange.

Title	Untargeted Proteomics to Assess the Impact of Moderate Hyperoxia on Patient Sample-derived Neonatal Airway Epithelial Cells in 3D Culture
Description	Neonatal Tracheal Aspirate-derived Airway Epithelial Cells (nTAECs) were isolated utilizing fresh tracheal suction aspirates from preterm (born at 23-28wks gestational age) and term infants (born at 37-42wks gestation). Included infants (especially term infants) did not have significant lung disease at the time of collection. Isolated cells were plated in 804g-cell-derived matrix coated T25, then expanded to a T75 flask and subsequently cultured in transwell inserts (0.4μm size pore) at 105 live cells/insert under air-liquid interface (ALI). Basal nTAECs have the capacity to differentiate into the full repertoire of mature airway epithelial lineages (ciliated, secretory club and mucus secreting goblet cells) during ALI culture. Cells were exposed to 60% O2 or room air control (21% O2) for 7 days (ALI day 7 to 14). On ALI day 14, cells will be harvested from the transwells and prepared for proteomic analysis using Data-independent acquisition (DIA) proteomics analysis. The samples will undergo several processing steps including chloroform/methanol extraction with trypsin digestion, Orbitrap Exploris 480 mass spectrometry analysis and eventual library construction and bioinformatics analysis. Utilizing 5 donor samples for each group, the proteomic workflow compared 4 exposure groups: a) Term nTAECs in Normoxia, b) Term nTAECs Hyperoxia, c) Preterm nTAECs in Normoxia, d) Preterm nTAECs in Hyperoxia.
HostingRepository	PRIDE
AnnounceDate	2026-03-02
AnnouncementXML	Submission_2026-03-01_17:48:19.124.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064170
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dennis Province
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-21 14:40:34	ID requested
⏵ 1	2026-03-01 17:48:20	announced

10.1152/ajplung.00205.2025;

10.6019/PXD064170;

Ganguly A, Carter CM, Rivera Negron A, Zhong H, Moreira A, Walters MS, Rogers LK, Prakash YS, Tipple TE, Richardson A, Quantitative proteomics links mitochondrial dysfunction to metabolic changes and epithelial differentiation defects in hyperoxia-exposed neonatal airway cells. Am J Physiol Lung Cell Mol Physiol, 330(1):L87-L103(2026) [pubmed]

submitter keyword: airway epithelium,neonate, tracheal aspirate, bronchopulmonary dysplasia

Abhrajit Ganguly
contact affiliation	University of Oklahoma Health Sciences Center
contact email	abhrajit-ganguly@ouhsc.edu
lab head
Dennis Province
contact affiliation	UAMS
contact email	dprovince@uams.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD064170

PRIDE project URI

• PRIDE
  1. PXD064170
     1. Label: PRIDE project
     2. Name: Untargeted Proteomics to Assess the Impact of Moderate Hyperoxia on Patient Sample-derived Neonatal Airway Epithelial Cells in 3D Culture