PXD064111

PXD064111 is an original dataset announced via ProteomeXchange.

Title	Lipid nanotubes unmask neutrophils for complement attack
Description	Neutrophils, critical components of innate immunity, undergo significant morphological changes during phagocytosis. In this study, we demonstrate that neutrophils exposed to shear stress generate lipid nanotubes (NTs) with a unique composition. Compared to the proteome of whole neutrophils, NTs notably lack cytoskeletal elements and the complement-inhibiting transmembrane protein CD46. Consequently, these NTs are recognized by the complement system and selectively opsonized the complement component C3b. Biophysical characterization of NTs confirmed that their integrity relies on lipid-lipid interactions and that they pinch off from neutrophils to form NT-derived vesicles (NTDVs). We detected C3b+ NTDVs in plasma from patients and animal models experiencing diverse inflammatory conditions, including metastatic melanoma, vasculitis, and polytrauma. Further functional experiments indicate that resting neutrophils phagocytose complement-opsonized NTDVs, leading to cell activation, including the production of reactive oxygen species (ROS). In conclusion, our data suggest a significant role for neutrophil-derived NTs in a wide range of inflammatory diseases and reveal a previously unknown mode of cell-cell communication.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:17:23.975.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bente Siebels
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-20 08:10:42	ID requested
⏵ 1	2026-09-06 16:17:24	announced

10.1182/blood.2025029974;

Liu X, Wang Y, Bauer AT, Marki A, Krebs CF, H, ä, ffner K, Siebels B, Schl, ü, ter H, Hildebrand F, Halbgebauer R, Huber-Lang M, Ley K, Schneider MF, Schneider SW, Gorzelanny C, Lipid nanotubes unmask neutrophils for complement attack. Blood, 147(12):1338-1350(2026) [pubmed]

submitter keyword: complement system, shear stress, extracellular vesicles,Neutrophils, polytrauma, vasculitis

Christian Gorzelanny
contact affiliation	Department of Dermatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
contact email	c.gorzelanny@uke.de
lab head
Bente Siebels
contact affiliation	Section for Mass spectrometry and Proteomics, University Medical Center Hamburg-Eppendorf
contact email	b.siebels@uke.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD064111

PRIDE project URI

• PRIDE
  1. PXD064111
     1. Label: PRIDE project
     2. Name: Lipid nanotubes unmask neutrophils for complement attack