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PXD064105

PXD064105 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGNPS Purified Recombinant His-HvGKEc Band in Gel
DescriptionRecombinant H. volcanii Glycerol Kinase expressed in E.coli BL21 DE3 (HvGKEc) using the plasmid pET15b+glpK in LB Amp. The protein was initially eluted in 50mM HEPES buffer, 2M NaCl. The sample was resuspended in 2X laemmli sample buffer and run in the SDS gel using 1X SDS buffer. The sampled was fixed, the gel was stained with bio-safe commassie overnight, and the band was analyzed.
HostingRepositoryMassIVE
AnnounceDate2025-10-27
AnnouncementXMLSubmission_2025-10-27_06:22:38.375.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarol
SpeciesList scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800; scientific name: Escherichia coli BL21(DE3); NCBI TaxID: 469008;
ModificationListNo PTMs are included in the dataset
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-05-20 06:50:44ID requested
12025-10-27 06:22:38announced
Publication List
no publication
Keyword List
submitter keyword: recombinant glycerol kinase, Haloferax volcanii, Escherichia coli, DatasetType:Proteomics
Contact List
Julie A. Maupin-Furlow
contact affiliationUniversity of Florida
contact emailjmaupin@ufl.edu
lab head
Karol
contact affiliationUniversity of Florida
contact emailsanchezkarol00@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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