PXD064096

PXD064096 is an original dataset announced via ProteomeXchange.

Title	Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins
Description	This project investigates the thermal stability of isolated mitochondrial proteins from Arabidopsis thaliana cell cultures following L-cysteine treatment, using Thermal Proteome Profiling (TPP). Shotgun proteomics was employed to identify and quantify proteins after thermal treatment. Quantitative data (iBAQ values) were subsequently analyzed using a custom R script (available on GitHub: “MeCuP”) to identify candidate proteins that exhibit changes in thermal stability due to interaction with L-cysteine.
HostingRepository	PRIDE
AnnounceDate	2025-05-29
AnnouncementXML	Submission_2025-05-29_01:37:37.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Björn Heinemann
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-20 02:56:42	ID requested
⏵ 1	2025-05-29 01:37:38	announced

10.1101/2025.05.23.655777;

submitter keyword: Thermal Proteome Profiling (TPP), Arabidopsis thaliana, Mitochondria, Melting Temperatures, timsTOF-HT,Shotgun Proteomics, Cell Culture, Melting Curves

Prof. Dr. Tatjana Hildebrandt
contact affiliation	University of Cologne CEPLAS/ Institute for Plant Sciences Plant Metabolic Biochemistry Lab Zülpicher Straße 47a D-50674 Cologne Germany
contact email	T.hildebrandt@uni-koeln.de
lab head
Björn Heinemann
contact affiliation	Universität zu Köln Abteilung Pflanzenstoffwechselbiochemie Institut für Pflanzenwissenschaften
contact email	bjoern.heinemann@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD064096

PRIDE project URI

• PRIDE
  1. PXD064096
     1. Label: PRIDE project
     2. Name: Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins