PXD064096 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins |
Description | This project investigates the thermal stability of isolated mitochondrial proteins from Arabidopsis thaliana cell cultures following L-cysteine treatment, using Thermal Proteome Profiling (TPP). Shotgun proteomics was employed to identify and quantify proteins after thermal treatment. Quantitative data (iBAQ values) were subsequently analyzed using a custom R script (available on GitHub: “MeCuP”) to identify candidate proteins that exhibit changes in thermal stability due to interaction with L-cysteine. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-29 |
AnnouncementXML | Submission_2025-05-29_01:37:37.983.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Björn Heinemann |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF HT |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-05-20 02:56:42 | ID requested | |
⏵ 1 | 2025-05-29 01:37:38 | announced | |
Publication List
Keyword List
submitter keyword: Thermal Proteome Profiling (TPP), Arabidopsis thaliana, Mitochondria, Melting Temperatures, timsTOF-HT,Shotgun Proteomics, Cell Culture, Melting Curves |
Contact List
Prof. Dr. Tatjana Hildebrandt |
contact affiliation | University of Cologne CEPLAS/ Institute for Plant Sciences Plant Metabolic Biochemistry Lab Zülpicher Straße 47a D-50674 Cologne Germany |
contact email | T.hildebrandt@uni-koeln.de |
lab head | |
Björn Heinemann |
contact affiliation | Universität zu Köln
Abteilung Pflanzenstoffwechselbiochemie
Institut für Pflanzenwissenschaften |
contact email | bjoern.heinemann@uni-koeln.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD064096
- Label: PRIDE project
- Name: Thermal Proteome Profiling (TPP) of l-Cysteine–Treated Arabidopsis Mitochondrial Proteins